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Visualizing the inner life of microbes: practices of multi-color single-molecule localization microscopy in microbiology

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Vojnovic,  Ilijana
Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Endesfelder,  Ulrike
Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

Vojnovic, I., Winkelmeier, J., & Endesfelder, U. (2019). Visualizing the inner life of microbes: practices of multi-color single-molecule localization microscopy in microbiology. Biochemical Society Transactions, 47(4), 1041-1065. doi:10.1042/BST20180399.


Cite as: https://hdl.handle.net/21.11116/0000-0008-BF06-B
Abstract
In this review, we discuss multi-color single-molecule imaging and
tracking strategies for studying microbial cell biology. We first
summarize and compare the methods in a detailed literature review of
published studies conducted in bacteria and fungi. We then introduce a
guideline on which factors and parameters should be evaluated when
designing a new experiment, from fluorophore and labeling choices to
imaging routines and data analysis. Finally, we give some insight into
some of the recent and promising applications and developments of these
techniques and discuss the outlook for this field.