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Cohesin and condensin extrude DNA loops in a cell-cycle dependent manner.

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Golfier,  Stefan
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Quail,  Thomas
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Brugués,  Jan
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Golfier, S., Quail, T., Kimura, H., & Brugués, J. (2020). Cohesin and condensin extrude DNA loops in a cell-cycle dependent manner. eLife, 9: e53885. doi:10.7554/eLife.53885.


Cite as: https://hdl.handle.net/21.11116/0000-0008-A288-7
Abstract
Loop extrusion by structural maintenance of chromosomes complexes (SMCs) has been proposed as a mechanism to organize chromatin in interphase and metaphase. However, the requirements for chromatin organization in these cell cycle phases are different, and it is unknown whether loop extrusion dynamics and the complexes that extrude DNA also differ. Here, we used Xenopus egg extracts to reconstitute and image loop extrusion of single DNA molecules during the cell cycle. We show that loops form in both metaphase and interphase, but with distinct dynamic properties. Condensin extrudes DNA loops non-symmetrically in metaphase, whereas cohesin extrudes loops symmetrically in interphase. Our data show that loop extrusion is a general mechanism underlying DNA organization, with dynamic and structural properties that are biochemically regulated during the cell cycle.