Label-free Redox Imaging of Patient-Derived Organoids Using Selective Plane 
Illumination Microscopy.

Favreau, Peter F.; He, Jiaye; Gil, Daniel A.; Deming, Dustin A.; Huisken, Jan; Skala, Melissa C.

doi:10.1364/BOE.389164

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Label-free Redox Imaging of Patient-Derived Organoids Using Selective Plane Illumination Microscopy.

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He, Jiaye
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Huisken, Jan
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Favreau, P. F., He, J., Gil, D. A., Deming, D. A., Huisken, J., & Skala, M. C. (2020). Label-free Redox Imaging of Patient-Derived Organoids Using Selective Plane Illumination Microscopy. Biomedical Optics Express, 11(5), 2591-2606. doi:10.1364/BOE.389164.

Cite as: https://hdl.handle.net/21.11116/0000-0008-A2DC-9

Abstract

High-throughput drug screening of patient-derived organoids offers an attractive platform to determine cancer treatment efficacy. Here, selective plane illumination microscopy (SPIM) was used to determine treatment response in organoids with endogenous fluorescence from the metabolic coenzymes NAD(P)H and FAD. Rapid 3-D autofluorescence imaging of colorectal cancer organoids was achieved. A quantitative image analysis approach was developed to segment each organoid and quantify changes in endogenous fluorescence caused by treatment. Quantitative analysis of SPIM volumes confirmed the sensitivity of patient-derived organoids to standard therapies. This proof-of-principle study demonstrates that SPIM is a powerful tool for high-throughput screening of organoid treatment response.