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Diphenylamine-2-carboxylate, a blocker of the Cl-conductive pathway in Cl-transporting epithelia

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di Stefano,  Antonio
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Wittner,  Monika
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Schlatter,  Eberhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Greger,  Rainer
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

di Stefano, A., Wittner, M., Schlatter, E., Lang, H., Englert, H., & Greger, R. (1985). Diphenylamine-2-carboxylate, a blocker of the Cl-conductive pathway in Cl-transporting epithelia. Pflügers Archiv: European Journal of Physiology, 405(Suppl 1), S95-S100. doi:10.1007/BF00581787.


Cite as: https://hdl.handle.net/21.11116/0000-0008-A50D-0
Abstract
The present study examines the effects of diphenylamine-2-carboxylate (DPC) in Cl-transporting epithelia. This substance blocks reversibly the Cl-conductance present under normal circumstances in the basolateral membrane of the thick ascending limb of the loop of Henle (TAL) and in the apical membrane of shark rectal gland tubules (RGT). This leads to a reduction in active NaCl reabsorption (TAL) and NaCl secretion (RGT) respectively, as measured by the equivalent short circuit current. The cells hyperpolarize as the membrane voltage drifts from the control value (some compromise between the chemical potential of Cl and K+) towards the chemical potential of Cl and K+. The resistance of the basolateral (TAL) or apical membrane (RGT) increases and this leads to a moderate increase in transepithelial resistance. In addition, the Cl-concentration step induced membrane voltage changes, which can be produced under control conditions, disappear in the presence of the blocker. Finally, experiments in excised membrane patches indicate that this substance inhibits the single current events of individual Cl-channels.