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Micropuncture studies on the pancreas of the rabbit

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Schulz,  Irene
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Yamagata,  Akifusa
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Weske,  Monika
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Schulz, I., Yamagata, A., & Weske, M. (1969). Micropuncture studies on the pancreas of the rabbit. Pflügers Archiv: European Journal of Physiology, 308, 277-290. doi:10.1007/BF00586559.


Cite as: https://hdl.handle.net/21.11116/0000-0008-B1FC-4
Abstract
Micropuncture and microperfusion experiments were performed at the exposed pancreas of rabbits. In the free-flow samples Cl concentration was determined, while in the stopped flow microperfusion samples the dilution of a plasma sample injected into the duct lumen was determined by measuring the change of Cl and labeled inulin concentrations. Following results were obtained: 1. Within 3–6 min a plasma sample injected into interlobar ducts of unstimulated glands increased in volume by a factor of 1.15 by the admixture of secreted fluid. The corresponding Cl concentration in the secreted fluid was in the mean 107 meq/l. After secretin stimulation the volume increased 2.33 fold and the mean Cl concentration of the secreted fluid was 52 meq/l. 2. A higher Cl concentration was measured along the entire duct under free-flow conditions during winter than during sommer. In each of both experimental series the Cl concentration decreased along the entire duct system when the gland was stimulated, but increased in the unstimulated gland. 3. The Cl concentration in samples obtained from the acini (probably from the centroacinar and adjacent intralobular duct) remained unchanged after secretin stimulation, while it decreased in the mean by 17 meq/l after pancreozymin stimulation. 4. Transtubular potential differences in all duct segments ranged between 2 and 6 mV (lumen negative).
From the results in can be concluded that bicarbonate and water are secreted within the entire duct system. The Cl concentration in the secreted fluid decreases and the HCO3 and Cl occurs in the ducts at small flow rates. Secretin does not change HCO3 concentration in the duct segments near the acini but that of more distal duct segments. A different, season dependent factor, influences the level of the Cl and HCO3 concentrations of the pancreas. Measurements of potential differences support the hypothesis that ductular secretion consists of an active HCO3 transport in addition to active Na+ transport postulated by others.