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Effect of La3+ on secretagogue-induced Ca2+ fluxes in rat isolated pancreatic acinar cells

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Wakasugi,  Hideyuki
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Stolze,  Hans H.
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Haase,  Winfried
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Schulz,  Irene
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Wakasugi, H., Stolze, H. H., Haase, W., & Schulz, I. (1981). Effect of La3+ on secretagogue-induced Ca2+ fluxes in rat isolated pancreatic acinar cells. American Journal of Physiology-Gastrointestinal and Liver Physiology, 240(4), G281-G289. doi:10.1152/ajpgi.1981.240.4.G281.


Cite as: http://hdl.handle.net/21.11116/0000-0008-B00C-4
Abstract
Addition of 0.1 mmol/l of La2+ to pancreatic acinar cells increased both the rate and extent of 45Ca2+ uptake. Addition of 0.3 mmol/l La2+ did not change cellular 45Ca2+, whereas 1, 2, and 5 mmol/l gradually decreased it. If carbamylcholine (CCh) or the octapeptide of cholecystokinin-pancreozymin (CCK-OP) was added in the presence of low La3+ concentrations (0.1 and 0.3 mmol/l) to 45Ca2+-equilibrated cells, secretagogue-induced 45Ca2+ release-reuptake was not inhibited. At 1 and 2 mol/l of La3+, however, secretagogue-induced 45Ca2+ release was abolished, whereas uptake of 45Ca2+ was still increased by CCK-OP and CCh. At 5 mmol/l La3+, the effects of secretagogues were completely abolished. In the presence of 2 mmol/l La3+, the atropine-induced vCa2+ uptake in CCh-pretreated cells and the dibutyryl guanosine 3',5'-cyclic monophosphate-induced 45Ca2+ uptake in CCK-OP-pretreated cells were highly reduced. The data are interpreted to support our assumption that CCK-OP and CCh increase the plasma membrane permeability to Ca2+ in pancreatic acinar cells in addition to their action to initiate release of CA2+ from an intracellular Ca2+ trigger pool.