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Journal Article

Isolierung und enzymatische Charakterisierung einer Bürstensaumfraktion der Rattenniere / Isolation and enzymatic characterisation of the brushborder-fraction of the rat kidney

MPS-Authors
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Kinne,  Rolf
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Kinne-Saffran,  Evamaria
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Kinne, R., & Kinne-Saffran, E. (1969). Isolierung und enzymatische Charakterisierung einer Bürstensaumfraktion der Rattenniere / Isolation and enzymatic characterisation of the brushborder-fraction of the rat kidney. Pflügers Archiv: European Journal of Physiology, 308, 1-15. doi:10.1007/BF00588029.


Cite as: https://hdl.handle.net/21.11116/0000-0008-B1A3-7
Abstract
The isolation of the brushborder-fraction is achieved by a fractional centrifugation in an isotonic sucrose medium followed by a separation in a sucrose density-gradient. The bruschborder-fragments sediment in the range of the density d 1.16/d 1.17. The electronmicroscopic investigation shows spherical fragments with well preserved microvilli. The specific activity of the alkaline phosphatase is twelve times higher than in the whole homogenat. The activity of the glutamate-dehydrogenase, lactate-dehydrogenase and the glucose-6-phosphatase is reduced. The (Na++K+)-stimulated, Ouabain sensitive ATP-phosphohydrolase is enriched by the factor four, the potassium-stimulated neutral p-nitrophenyl-phosphatase shows a similar behaviour.