Restored DNA-binding of the cAMP-CRP activator complex reestablishes negative 
regulation by the CytR repressor in the deoP2 promoter in Escherichia coli

Sogaard-Andersen, Lotte; Valentin-Hansen, P.

doi:10.1007/BF00293824

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Restored DNA-binding of the cAMP-CRP activator complex reestablishes negative regulation by the CytR repressor in the deoP2 promoter in Escherichia coli

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Sogaard-Andersen, L., & Valentin-Hansen, P. (1991). Restored DNA-binding of the cAMP-CRP activator complex reestablishes negative regulation by the CytR repressor in the deoP2 promoter in Escherichia coli. MOLECULAR & GENERAL GENETICS, 231(1), 76-80. doi:10.1007/BF00293824.

Cite as: https://hdl.handle.net/21.11116/0000-0008-D671-7

Abstract

We have investigated in vivo the coupling between CytR regulation of the deoP2 promoter in Escherichia coli and the DNA-binding specificity of the cAMP-CRP (cAMP receptor protein) complex in order to obtain a more detailed picture of the role played by cAMP-CRP in CytR regulation. By introducing CRP proteins that exhibit an altered DNA binding specificity into a strain containing a mutant deoP2 promoter in which cAMP-CRP activation was decreased and CytR regulation completely abolished, we show that CytR regulation of this promoter can be reestablished by restored the DNA binding of the cAMP-CRP complex. Hence, CytR regulation of deoP2 can be modulated by simply varying DNA binding of cAMP-CRP. These data confirm the crucial role played by the cAMP-CRP activator complex in CytR regulation of the deoP2 promoter.