English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Direct visualization of amlodipine intervention into living cells by means of fluorescence microscopy

MPS-Authors
/persons/resource/persons262937

Quentin,  C.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons220611

Gerasimaite,  R.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons203920

Lukinavičius,  G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons14832

Belov,  V. N.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons36443

Mitronova,  G.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (public)

3328303.pdf
(Publisher version), 4MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Quentin, C., Gerasimaite, R., Freidzon, A., Atabekyan, L. S., Lukinavičius, G., Belov, V. N., et al. (2021). Direct visualization of amlodipine intervention into living cells by means of fluorescence microscopy. Molecules, 26(10): 2997. doi:10.3390/molecules26102997.


Cite as: http://hdl.handle.net/21.11116/0000-0008-C468-6
Abstract
Amlodipine, a unique long-lasting calcium channel antagonist and antihypertensive drug, has weak fluorescence in aqueous solutions. In the current paper, we show that direct visualization of amlodipine in live cells is possible due to the enhanced emission in cellular environment. We examined the impact of pH, polarity and viscosity of the environment as well as protein binding on the spectral properties of amlodipine in vitro, and used quantum chemical calculations for assessing the mechanism of fluorescence quenching in aqueous solutions. The confocal fluorescence microscopy shows that the drug readily penetrates the plasma membrane and accumulates in the intracellular vesicles. Visible emission and photostability of amlodipine allow confocal time-lapse imaging and the drug uptake monitoring.