English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Three-dimensional superresolution fluorescence microscopy maps the variable molecular architecture of the nuclear pore complex

MPS-Authors
/persons/resource/persons232071

Beck,  Martin       
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;
Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

mbc.e20-11-0728.pdf
(Any fulltext), 3MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Sabinina, V. J., Hossain, M. J., Hériché, J.-K., Hoess, P., Nijmeijer, B., Mosalaganti, S., et al. (2021). Three-dimensional superresolution fluorescence microscopy maps the variable molecular architecture of the nuclear pore complex. Molecular Biology of the Cell, 32(17), 1523-1533. doi:10.1091/mbc.E20-11-0728.


Cite as: https://hdl.handle.net/21.11116/0000-0008-C811-3
Abstract
Nuclear pore complexes (NPCs) are large macromolecular machines that mediate the traffic between the nucleus and the cytoplasm. In vertebrates, each NPC consists of ∼1000 proteins, termed nucleoporins, and has a mass of over 100 MDa. While a pseudo-atomic static model of the central scaffold of the NPC has recently been assembled by integrating data from isolated proteins and complexes, many structural components still remain elusive due to the enormous size and flexibility of the NPC. Here, we explored the power of 3D super-resolution microscopy combined with computational classification and averaging to explore the 3D structure of the NPC in single human cells. We show that this approach can build the first integrated 3D structural map containing both central as well as peripheral NPC subunits with molecular specificity and nanoscale resolution. Our unbiased classification of over ten thousand individual NPCs indicates that the nuclear ring and the nuclear basket can adopt different conformations. Our approach opens up the exciting possibility to relate different structural states of the NPC to function in situ.