Toward deep biophysical cytometry: prospects and challenges

Lee, Kelvin C.M.; Guck, Jochen; Goda, Keisuke; Tsia, Kevin K.

doi:10.1016/j.tibtech.2021.03.006

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Toward deep biophysical cytometry: prospects and challenges

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Guck, Jochen
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Friedrich-Alexander-Universität Erlangen-Nürnberg, Department Physik;

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Citation

Lee, K. C., Guck, J., Goda, K., & Tsia, K. K. (2021). Toward deep biophysical cytometry: prospects and challenges. Trends in Biotechnology, 39(12), 1249-1262. doi:10.1016/j.tibtech.2021.03.006.

Cite as: https://hdl.handle.net/21.11116/0000-0008-D499-C

Abstract

Recent advances in biophysical cytometry now make it possible to recapitulate cellular heterogeneity at the levels of throughput, precision, specificity, and sensitivity that were once inconceivable. Technological developments in state-of-the-art biophysical cytometry include single-cell mass assays, cell traction force assays, deformability and impedance cytometry, and label-free imaging cytometry. Next-generation biophysical cytometry could be more comprehensive and information-rich by exploring multimodal integration, such as simultaneous read-out of cell mass, stiffness, and morphology. How molecular signatures translate into cellular biophysical properties is not fully understood. Advanced techniques involving microfluidics, imaging, and deep learning could investigate this link. Standardizing the protocols and datasets of biophysical cytometry will be crucial to ensure wide dissemination.