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Journal Article

Mouse multipotent progenitor 5 cells are located at the interphase between hematopoietic stem and progenitor cells

MPS-Authors

Romero-Mulero,  Mari Carmen
Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Schönberger,  Katharina
Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Jäcklein,  Karin
Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Cabezas-Wallscheid,  Nina
Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

External Resource

https://ashpublications.org/blood/article/137/23/3218/475584/Mouse-multipotent-progenitor-5-cells-are-located
(Publisher version)

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Citation

Sommerkamp, P., Romero-Mulero, M. C., Narr, A., Ladel, L., Hustin, L., Schönberger, K., et al. (2021). Mouse multipotent progenitor 5 cells are located at the interphase between hematopoietic stem and progenitor cells. Blood, 137, 3218-3224. doi:10.1182/blood.2020007876.


Cite as: https://hdl.handle.net/21.11116/0000-0008-D99C-4
Abstract
Hematopoietic stem cells (HSCs) and distinct multipotent progenitor (MPP) populations (MPP1-4) contained within the Lin−Sca-1+c-Kit+ (LSK) compartment have previously been identified using diverse surface-marker panels. Here, we phenotypically define and functionally characterize MPP5 (LSK CD34+CD135−CD48−CD150−). Upon transplantation, MPP5 supports initial emergency myelopoiesis followed by stable contribution to the lymphoid lineage. MPP5, capable of generating MPP1-4 but not HSCs, represents a dynamic and versatile component of the MPP network. To characterize all hematopoietic stem and progenitor cells, we performed RNA-sequencing (RNA-seq) analysis to identify specific transcriptomic landscapes of HSCs and MPP1-5. This was complemented by single-cell RNA-seq analysis of LSK cells to establish the differentiation trajectories from HSCs to MPP1-5. In agreement with functional reconstitution activity, MPP5 is located immediately downstream of HSCs but upstream of the more committed MPP2-4. This study provides a comprehensive analysis of the LSK compartment, focusing on the functional and molecular characteristics of the newly defined MPP5 subset.