Single-molecule imaging reveals that Z-ring condensation is essential for cell 
division in Bacillus subtilis.

Squyres, Georgia R; Holmes, Matthew J; Barger, Sarah R; Pennycook, Betheney R; Ryan, Joel; Yan, Victoria T; Garner, Ethan C

doi:10.1038/s41564-021-00878-z

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Single-molecule imaging reveals that Z-ring condensation is essential for cell division in Bacillus subtilis.

MPS-Authors

/persons/resource/persons263352

Yan, Victoria T
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Squyres, G. R., Holmes, M. J., Barger, S. R., Pennycook, B. R., Ryan, J., Yan, V. T., et al. (2021). Single-molecule imaging reveals that Z-ring condensation is essential for cell division in Bacillus subtilis. Nature microbiology, 6(5), 553-562. doi:10.1038/s41564-021-00878-z.

Cite as: https://hdl.handle.net/21.11116/0000-0008-DB0B-6

Abstract

Although many components of the cell division machinery in bacteria have been identified1,2, the mechanisms by which they work together to divide the cell remain poorly understood. Key among these components is the tubulin FtsZ, which forms a Z ring at the midcell. FtsZ recruits the other cell division proteins, collectively called the divisome, and the Z ring constricts as the cell divides. We applied live-cell single-molecule imaging to describe the dynamics of the divisome in detail, and to evaluate the individual roles of FtsZ-binding proteins (ZBPs), specifically FtsA and the ZBPs EzrA, SepF and ZapA, in cytokinesis. We show that the divisome comprises two subcomplexes that move differently: stationary ZBPs that transiently bind to treadmilling FtsZ filaments, and a moving complex that includes cell wall synthases. Our imaging analyses reveal that ZBPs bundle FtsZ filaments together and condense them into Z rings, and that this condensation is necessary for cytokinesis.