English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse
  1. View item / 
  2. Item Summary

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

A method for the temperature-controlled extraction of DNA from ancient bones

MPS-Authors
/persons/resource/persons239847

Essel,  Elena       
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

/persons/resource/persons181051

Korlević,  Petra
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

/persons/resource/persons72846

Meyer,  Matthias
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

External Resource

https://www.protocols.io/view/a-method-for-the-temperature-controlled-extraction-bje5kjg6
(Supplementary material)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

Essel_Method_BioTechniques_2021.pdf
(Publisher version), 298KB

Supplementary Material (public)
There is no public supplementary material available
Citation

Essel, E., Korlević, P., & Meyer, M. (2021). A method for the temperature-controlled extraction of DNA from ancient bones. Biotechniques, 71(1), 383-387. doi:10.2144/btn-2021-0025.


Cite as: https://hdl.handle.net/21.11116/0000-0008-E0F5-6
Abstract
We here provide a protocol for the decontamination of ancient bones and teeth that is based on a temperature-controlled, sequential release of DNA. DNA can be extracted from all fractions generated with this method and the fraction with the highest proportion of endogenous DNA identified for further analysis. The protocol proceeds through repeated incubation of the sample powder in phosphate buffer at 37, 60 and 90 °C, followed by the complete lysis of the residual sample powder. As DNA is denatured at high temperature, subsequent DNA extraction and library preparation has to be performed using methods optimized for single-stranded DNA.