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Journal Article

Arrangement of tRNAs in Pre- and Posttranslocational Ribosomes Revealed by Electron Cryomicroscopy

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Stark,  Holger
Inorganic Chemistry, Fritz Haber Institute, Max Planck Society;

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Orlova,  Elena V.
Fritz Haber Institute, Max Planck Society;
Imperial College of Science, Medicine and Technology, Department of Biochemistry;

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Heel,  Marin van
Fritz Haber Institute, Max Planck Society;
Imperial College of Science, Medicine and Technology, Department of Biochemistry;

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Citation

Stark, H., Orlova, E. V., Rinke-Appel, J., Jünke, N., Mueller, F., Rodnina, M., et al. (1997). Arrangement of tRNAs in Pre- and Posttranslocational Ribosomes Revealed by Electron Cryomicroscopy. Cell, 88(1), 19-28. doi:10.1016/S0092-8674(00)81854-1.


Cite as: https://hdl.handle.net/21.11116/0000-0008-ECD5-E
Abstract
The three-dimensional structure of the translating 70S E. coli ribosome is presented in its two main conformations: the pretranslocational and the posttranslocational states. Using electron cryomicroscopy and angular reconstitution, structures at 20 Å resolution were obtained, which, when compared with our earlier reconstruction of “empty” ribosomes, showed densities corresponding to tRNA molecules—at the P and E sites for posttranslocational ribosomes and at the A and P sites for pretranslocational ribosomes. The P-site tRNA lies directly above the bridge connecting the two ribosomal subunits, with the A-site tRNA fitted snugly against it at an angle of ∼50°, toward the L7/L12 side of the ribosome. The E-site tRNA appears to lie between the side lobe of the 30S subunit and the L1 protuberance.