In vitro studies of cell proliferation in tumours—II: Characteristics of a 
standardised in vitro system for the measurement of3H-thymidine incorporation 
into tissue explants

Rajewsky, Manfred F.

doi:10.1016/0014-2964(65)90061-7

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In vitro studies of cell proliferation in tumours—II: Characteristics of a standardised in vitro system for the measurement of ³H-thymidine incorporation into tissue explants

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Rajewsky, Manfred F.
Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Rajewsky, M. F. (1965). In vitro studies of cell proliferation in tumours—II: Characteristics of a standardised in vitro system for the measurement of ³H-thymidine incorporation into tissue explants. European Journal of Cancer, 1(3-4), 281-287. doi:10.1016/0014-2964(65)90061-7.

Cite as: https://hdl.handle.net/21.11116/0000-0008-EC08-6

Abstract

A standardized in vitro system for the measurement of ³H-thymidine incorporation into tissue explants is described, and by its use a good correlation is demonstrated between relative tritium activity per mg wet wt and autoradiographic thymidine labelling index (L.I.). The value of this technique as a method of determining overall L.I.s is discussed, especially as regards tissues with a low L.I. or with a highly inhomogeneous distribution of DNA synthesizing nuclei, as often occurs in tumours.
The importance of the oxygen partial pressure in the incubated explants for the process of DNA synthesis and hence for the demonstration of ³H-labelled nuclei is considered both theoretically and on the basis of experimental data. Results of measurements on the kinetics of ³H-thymidine incorporation in the in vitro system are presented.