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Beginner’s guide to producing super-resolved images on a widefield fluorescence microscope

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Endesfelder,  U.
Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

Vojnovic, I., & Endesfelder, U. (2020). Beginner’s guide to producing super-resolved images on a widefield fluorescence microscope. The biochemist: the bulletin of the Biochemical Society, 42(4), 52-566. doi:10.1042/BIO20200045.


Cite as: http://hdl.handle.net/21.11116/0000-0008-EEB2-3
Abstract
The development of super-resolution microscopy techniques, which are able to achieve resolutions in the nanometre range and as such allow the visualization of subcellular structures and dynamics, has considerably expanded the possibilities of fluorescence microscopy in the life sciences. While a majority of these techniques require highly specialized hardware, single-molecule localization microscopy (SMLM) can be implemented on conventional widefield fluorescence microscopes. Here, we describe what technical upgrades are necessary and discuss some of the difficulties that can be encountered during sample preparation and imaging.