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Journal Article

A color-shifting near-infrared fluorescent aptamer-fluorophore module for live-cell RNA imaging


Wang,  Lu
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

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Zhang, J., Wang, L., Jäschke, A., & Sunbul, M. (2021). A color-shifting near-infrared fluorescent aptamer-fluorophore module for live-cell RNA imaging. Angewandte Chemie International Edition, 1-8. doi:10.1002/anie.202107250.

Cite as: http://hdl.handle.net/21.11116/0000-0008-F3BD-1
Fluorescent light-up RNA aptamers (FLAPs) have become promising tools for visualizing RNAs in living cells. Specific binding of FLAPs to their non-fluorescent cognate ligands results in a dramatic fluorescence increase, thereby allowing RNA imaging. Here, we present a color-shifting aptamer-fluorophore system, where the free dye is cyan fluorescent and the aptamer-dye complex is near-infrared (NIR) fluorescent. Unlike other reported FLAPs, this system enables ratiometric RNA imaging. To design the color-shifting system, we synthesized a series of environmentally sensitive benzopyrylium-coumarin hybrid fluorophores which exist in equilibrium between a cyan fluorescent spirocyclic form and a NIR fluorescent zwitterionic form. As an RNA tag, we evolved a 38-nucleotide aptamer that selectively binds the zwitterionic forms with nanomolar affinity. We used this system as a light-up RNA marker to image mRNAs in the NIR region and demonstrated its utility in ratiometric analysis of target RNAs expressed at different levels in single cells.