In situ cryo-electron tomography reveals gradient organization of ribosome 
biogenesis in intact nucleoli

Erdmann, Philipp S.; Hou, Zhen; Klumpe, Sven; Khavnekar, Sagar; Beck, Florian; Wilfling, Florian; Plitzko, Jürgen M.; Baumeister, Wolfgang

doi:10.1038/s41467-021-25413-w

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli

MPS-Authors

Erdmann, Philipp S.
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;
Fondazione Human Technopole, Milano, Italy;

Hou, Zhen
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

Klumpe, Sven
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

Khavnekar, Sagar
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

Beck, Florian
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons172944

Wilfling, Florian
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;
Research Group Mechanisms of Cellular Quality Control, Max Planck Institute of Biophysics, Max Planck Society;

Plitzko, Jürgen M.
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

Baumeister, Wolfgang
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Erdmann, P. S., Hou, Z., Klumpe, S., Khavnekar, S., Beck, F., Wilfling, F., et al. (2021). In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli. Nature Communications, 12(1): 5364. doi:10.1038/s41467-021-25413-w.

Cite as: https://hdl.handle.net/21.11116/0000-0009-26E1-E

Abstract

Ribosomes comprise a large (LSU) and a small subunit (SSU) which are synthesized independently in the nucleolus before being exported into the cytoplasm, where they assemble into functional ribosomes. Individual maturation steps have been analyzed in detail using biochemical methods, light microscopy and conventional electron microscopy (EM). In recent years, single particle analysis (SPA) has yielded molecular resolution structures of several pre-ribosomal intermediates. It falls short, however, of revealing the spatiotemporal sequence of ribosome biogenesis in the cellular context. Here, we present our study on native nucleoli in Chlamydomonas reinhardtii, in which we follow the formation of LSU and SSU precursors by in situ cryo-electron tomography (cryo-ET) and subtomogram averaging (STA). By combining both positional and molecular data, we reveal gradients of ribosome maturation within the granular component (GC), offering a new perspective on how the liquid-liquid-phase separation of the nucleolus supports ribosome biogenesis.