Cloning and expression of a Clostridium cellobioparum cellulase gene and its 
excretion from Escherichia coli JM109

Shima, Seigo; Kato, Junichi; Igarashi, Yasuo; Kodama, Tohru

doi:10.1099/00207713-43-4-703

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Cloning and expression of a Clostridium cellobioparum cellulase gene and its excretion from Escherichia coli JM109

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引用

Shima, S., Kato, J., Igarashi, Y., & Kodama, T. (1989). Cloning and expression of a Clostridium cellobioparum cellulase gene and its excretion from Escherichia coli JM109. Journal of Fermentation and Bioengineering, 68(2), 75-78. doi:10.1099/00207713-43-4-703.

引用: https://hdl.handle.net/21.11116/0000-0009-4B8F-3

要旨

A cellulase gene of Clostridium cellobioparum was cloned on vector pUC19 to form a recombinant plasmid pCMC1 and expressed in Escherichia coli. The expressed enzyme showed carboxymethylcellulase activity but could not degrade Avicel. E. coli JM109 carrying pCMC1 excreted a large amount of cellulase into a culture medium. The C. cellobioparum DNA sequence in the pCMC1 directed the cellulase excretion.