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Journal Article

The prevalence and specificity of local protein synthesis during neuronal synaptic plasticity

MPS-Authors

Sun ,  Chao
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

Nold,  Andreas
Theory of neural dynamics Group, Max Planck Institute for Brain Research, Max Planck Society;
Institute of Experimental Epileptology and Cognition Research, Life and Brain Center, Universitätsklinikum Bonn;

Fusco,  Claudia
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

Rangaraju,  Vidhya
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

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Tchumatchenko,  Tatjana
Theory of neural dynamics Group, Max Planck Institute for Brain Research, Max Planck Society;
Institute of Experimental Epileptology and Cognition Research, Life and Brain Center, Universitätsklinikum Bonn;

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Schuman,  Erin M.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448450/
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sciadv.abj0790.pdf
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Citation

Sun, C., Nold, A., Fusco, C., Rangaraju, V., Tchumatchenko, T., Heilemann, M., et al. (2021). The prevalence and specificity of local protein synthesis during neuronal synaptic plasticity. Sci. Adv., 7(38): eabj0790. doi:10.1126/sciadv.abj0790.


Cite as: https://hdl.handle.net/21.11116/0000-0009-48CB-2
Abstract
To supply proteins to their vast volume, neurons localize mRNAs and ribosomes in dendrites and axons. While local protein synthesis is required for synaptic plasticity, the abundance and distribution of ribosomes and nascent proteins near synapses remain elusive. Here, we quantified the occurrence of local translation and visualized the range of synapses supplied by nascent proteins during basal and plastic conditions. We detected dendritic ribosomes and nascent proteins at single-molecule resolution using DNA-PAINT and metabolic labeling. Both ribosomes and nascent proteins positively correlated with synapse density. Ribosomes were detected at ~85% of synapses with ~2 translational sites per synapse; ~50% of the nascent protein was detected near synapses. The amount of locally synthesized protein detected at a synapse correlated with its spontaneous Ca2+ activity. A multifold increase in synaptic nascent protein was evident following both local and global plasticity at respective scales, albeit with substantial heterogeneity between neighboring synapses.