Crystallization and preliminary X‐ray diffraction studies of 
formylmethanofuran: Tetrahydromethanopterin formyltransferase from Methanopyrus 
kandleri

Shima, Seigo; Thauer, Rudolf K.; Michel, Hartmut; Ermler, Ulrich

doi:10.1002/(SICI)1097-0134(199609)26:1<118::AID-PROT12>3.0.CO;2-J

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Crystallization and preliminary X‐ray diffraction studies of formylmethanofuran: Tetrahydromethanopterin formyltransferase from Methanopyrus kandleri

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Shima, Seigo
Department-Independent Research Group Microbial Protein Structure, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps- Universität, Marburg;

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Thauer, Rudolf K.
Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps- Universität, Marburg;

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Citation

Shima, S., Thauer, R. K., Michel, H., & Ermler, U. (1996). Crystallization and preliminary X‐ray diffraction studies of formylmethanofuran: Tetrahydromethanopterin formyltransferase from Methanopyrus kandleri. Proteins: Structure, Function, and Bioinformatics, 26(1), 118-120. doi:10.1002/(SICI)1097-0134(199609)26:1<118:AID-PROT12>3.0.CO;2-J.

Cite as: https://hdl.handle.net/21.11116/0000-0009-4B3C-1

Abstract

Formylmethanofuran:tetrahydromethanopterin formyltransferase from the hyperthermophilic methanogenic Archaeon Methanopyrus kandleri (growth temperature optimum 98°C) was crystallized by vapor diffusion methods. Crystal form M obtained with 2‐methyl‐2,4‐pentanediol as precipitant displayed the space group P2₁ with unit cell parameters of a = 87.0 Å, b = 75.4 Å, c = 104.7 Å, and β = 113.9° and diffracted better than 2 Å resolution. Crystal form P grown from polyethylene glycol 8000 belonged to the space group /4₁22 and had unit cell parameters of 157.5 Å and 242.1 Å. Diffraction data to 1.73 Å were recorded. Crystal form S which was crystallized from (NH₄)₂SO₄in the space group /4₁22 with unit cell parameters of 151.3 Å and 249.5 Å diffracted at least to 2.2 Å resolution. All crystal forms probably have four molecules per asymmetric unit and are suitable for X‐ray structure analysis