Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes

Malik, Victoria Adelheid; Di Benedetto, Barbara; Jakovcevski, Mira

doi:10.1007/978-1-4939-9068-9_15

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Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes

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Jakovcevski, Mira
Dept. Stress Neurobiology and Neurogenetics, Max Planck Institute of Psychiatry, Max Planck Society;

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Malik, V. A., Di Benedetto, B., & Jakovcevski, M. (2019). Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes. In ASTROCYTES: METHODS AND PROTOCOLS (pp. 219-229).

Cite as: https://hdl.handle.net/21.11116/0000-0009-7726-7

Abstract

Chromatin immunoprecipitation (ChIP) in conjunction with qPCR or next generation sequencing (ChIPseq) is used to detect protein-DNA interaction. Typically, DNA bound to a protein of interest is captured with an antibody against this protein, and DNA is then purified from DNA-protein complexes. Here, we describe a native Chromatin immunoprecipitation (N-ChIP) approach which is an efficient ChIP method with high resolution for histone modifications and a number of transcription factors. This protocol has been tailored for cultured primary rat astrocytes, and we included the preparation of astrocytic cell cultures in this protocol.