A structure of the COPI coat and the role of coat proteins in membrane vesicle 
assembly

Dodonova, S. O.; Diestelkoetter-Bachert, P.; von Appen, A.; Hagen, W. J. H.; Beck, R.; Beck, M.; Wieland, F.; Briggs, John A. G.

doi:10.1126/science.aab1121

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Previous Next of 522778

Released

Journal Article

A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Dodonova, S. O., Diestelkoetter-Bachert, P., von Appen, A., Hagen, W. J. H., Beck, R., Beck, M., et al. (2015). A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly. Science, 349(6244), 195-198. doi:10.1126/science.aab1121.

Cite as: https://hdl.handle.net/21.11116/0000-0009-7165-6

Abstract

Transport of material within cells is mediated by trafficking vesicles that bud from one cellular compartment and fuse with another. Formation of a trafficking vesicle is driven by membrane coats that localize cargo and polymerize into cages to bend the membrane. Although extensive structural information is available for components of these coats, the heterogeneity of trafficking vesicles has prevented an understanding of how complete membrane coats assemble on the membrane. We combined cryo-electron tomography, subtomogram averaging, and cross-linking mass spectrometry to derive a complete model of the assembled coat protein complex I (COPI) coat involved in traffic between the Golgi and the endoplasmic reticulum. The highly interconnected COPI coat structure contradicted the current "adaptor-and-cage" understanding of coated vesicle formation.