Photoactivatable fluorescent dyes with hydrophilic caging groups and their use 
in multicolor nanoscopy

Butkevich, A. N.; Weber, M.; Cereceda Delgado, A.R.; Ostersehlt, L. M.; d'Este, E.; Hell, S. W.

doi:10.1021/jacs.1c09999

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学術論文

Photoactivatable fluorescent dyes with hydrophilic caging groups and their use in multicolor nanoscopy

MPS-Authors

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Butkevich, A. N.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons205203

Weber, M.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons266814

Cereceda Delgado, A.R.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons266816

Ostersehlt, L. M.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15210

Hell, S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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引用

Butkevich, A. N., Weber, M., Cereceda Delgado, A., Ostersehlt, L. M., d'Este, E., & Hell, S. W. (2021). Photoactivatable fluorescent dyes with hydrophilic caging groups and their use in multicolor nanoscopy. Journal of the American Chemical Society, 143(44), 18388-18393. doi:10.1021/jacs.1c09999.

引用: https://hdl.handle.net/21.11116/0000-0009-6EA1-6

要旨

We propose a series of fluorescent dyes with hydrophilic carbamate caging groups that undergo rapid photoactivation under UV (≤400 nm) irradiation but do not undergo spurious two-photon activation with high-intensity (visible or infrared) light of about twice the wavelength. The caged fluorescent dyes and labels derived therefrom display high water solubility and convert upon photoactivation into validated super-resolution and live-cell-compatible fluorophores. In combination with popular fluorescent markers, multiple (up to six)-color images can be obtained with stimulated emission depletion nanoscopy. Moreover, individual fluorophores can be localized with precision <3 nm (standard deviation) using MINSTED and MINFLUX techniques.