Fusion-induced growth of biomimetic polymersomes : behavior of 
poly(dimethylsiloxane)-poly(ethylene oxide) vesicles in saline solutions under 
high agitation

Marušič, Nika; Zhao, Ziliang; Otrin, Lado; Dimova, Rumiana; Ivanov, Ivan; Sundmacher, Kai

doi:10.1002/marc.202100712

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Fusion-induced growth of biomimetic polymersomes : behavior of poly(dimethylsiloxane)-poly(ethylene oxide) vesicles in saline solutions under high agitation

MPS-Authors

/persons/resource/persons230489

Zhao, Ziliang
Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

/persons/resource/persons121236

Dimova, Rumiana
Rumiana Dimova, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

Article.pdf
(出版社版), 4MB

付随資料 (公開)

There is no public supplementary material available

引用

Marušič, N., Zhao, Z., Otrin, L., Dimova, R., Ivanov, I., & Sundmacher, K. (2022). Fusion-induced growth of biomimetic polymersomes: behavior of poly(dimethylsiloxane)-poly(ethylene oxide) vesicles in saline solutions under high agitation. Macromolecular Rapid Communications, 43(5):. doi:10.1002/marc.202100712.

引用: https://hdl.handle.net/21.11116/0000-0009-96D0-2

要旨

Giant unilamellar vesicles serve as membrane models and primitive mockups of natural cells. With respect to the latter use, amphiphilic polymers can be used to replace phospholipids in order to introduce certain favorable properties, ultimately allowing for the creation of truly synthetic cells. These new properties also enable the employment of new preparation procedures that are incompatible with the natural amphiphiles. Whereas the growth of lipid compartments to micrometer dimensions has been well established, growth of their synthetic analogs remains underexplored. Here, we investigate in detail the influence of experimental parameters like salt type/concentration and magnitude of agitation on the fusion of nanometer-sized vesicles made of poly(dimethylsiloxane)-poly(ethylene oxide) graft copolymer (PDMS-g-PEO). To this end, we employ dynamic light scattering, microscopy and membrane mixing assays, and analyze the process at different time and length scales. We use this optimized method as an easy tool to obtain giant vesicles, equipped with membrane and cytosolic biomachinery, in the presence of salts at physiological concentrations.