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Journal Article

Repression of the Hox gene abd-A by ELAV-mediated Transcriptional Interference

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Carrasco Sala,  Judit
Department Independent Research Groups, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Hilgers,  Valérie
Department Independent Research Groups, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1009843
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Castro Alvarez et al. 2021.pdf
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Citation

Castro Alvarez, J. J., Revel, M., Carrasco Sala, J., Cléard, F., Pauli, D., Hilgers, V., et al. (2021). Repression of the Hox gene abd-A by ELAV-mediated Transcriptional Interference. PLoS Genetics, 17(11): e1009843. doi:10.1371/journal.pgen.1009843.


Cite as: https://hdl.handle.net/21.11116/0000-0009-8F63-7
Abstract
Intergenic transcription is a common feature of eukaryotic genomes and performs important and diverse cellular functions. Here, we investigate the iab-8 ncRNA from the Drosophila Bithorax Complex and show that this RNA is able to repress the transcription of genes located at its 3’ end by a sequence-independent, transcriptional interference mechanism. Although this RNA is expressed in the early epidermis and CNS, we find that its repressive activity is limited to the CNS, where, in wild-type embryos, it acts on the Hox gene, abd-A, located immediately downstream of it. The CNS specificity is achieved through a 3’ extension of the transcript, mediated by the neuronal-specific, RNA-binding protein, ELAV. Loss of ELAV activity eliminates the 3’ extension and results in the ectopic activation of abd-A. Thus, a tissue-specific change in the length of a ncRNA is used to generate a precise pattern of gene expression in a higher eukaryote.