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Proton detected solid-state NMR of membrane proteins at 28 Tesla (1.2 GHz) and 100 kHz magic-angle spinning

MPS-Authors
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Nimerovsky,  E.
Department of NMR Based Structural Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Tekwani Movellan,  K.
Research Group of Solid State NMR Spectroscopy-2, MPI for Biophysical Chemistry, Max Planck Society;

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Najbauer,  E.
Research Group of Solid State NMR Spectroscopy-2, MPI for Biophysical Chemistry, Max Planck Society;

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Xue,  K.
Research Group of Solid State NMR Spectroscopy-2, MPI for Biophysical Chemistry, Max Planck Society;

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Dervisoglu,  R.
Research Group of Solid State NMR Spectroscopy-2, MPI for Biophysical Chemistry, Max Planck Society;

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Giller,  K.
Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Griesinger,  C.
Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Becker,  S.
Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Andreas,  L. B.
Research Group of Solid State NMR Spectroscopy-2, MPI for Biophysical Chemistry, Max Planck Society;

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3355987.pdf
(Publisher version), 4MB

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Citation

Nimerovsky, E., Tekwani Movellan, K., Zhang, X. C., Forster, M. C., Najbauer, E., Xue, K., et al. (2021). Proton detected solid-state NMR of membrane proteins at 28 Tesla (1.2 GHz) and 100 kHz magic-angle spinning. Biomolecules, 11(5): 752. doi:10.3390/biom11050752.


Cite as: http://hdl.handle.net/21.11116/0000-0009-90C5-5
Abstract
The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1200 MHz. We find a consistent improvement in resolution that scales superlinearly with the increase in magnetic field for three of the four examples. In 3D and 4D spectra, which are now routinely acquired, this improvement indicates the ability to resolve at least 2 and 2.5 times as many signals, respectively.