Design Features to Accelerate the Higher-Order Assembly of DNA Origami on 
Membranes

Qutbuddin, Yusuf; Krohn, Jan-Hagen; Brüggenthies, Gereon A.; Stein, Johannes; Gavrilovic, Svetozar; Stehr, Florian; Schwille, Petra

doi:10.1021/acs.jpcb.1c07694

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Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes

MPS-Authors

Qutbuddin, Yusuf
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

Krohn, Jan-Hagen
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

Brüggenthies, Gereon A.
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Stein, Johannes
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

Gavrilovic, Svetozar
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Stehr, Florian
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Schwille, Petra
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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acs.jpcb.1c07694.pdf
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5722606.zip
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Citation

Qutbuddin, Y., Krohn, J.-H., Brüggenthies, G. A., Stein, J., Gavrilovic, S., Stehr, F., et al. (2021). Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes. The Journal of Physical Chemistry B, 125, 3181-13191. doi:10.1021/acs.jpcb.1c07694.

Cite as: https://hdl.handle.net/21.11116/0000-0009-9421-A

Abstract

Nanotechnology often exploits DNA origami nanostructures assembled into even larger superstructures up to micrometer sizes with nanometer shape precision. However, large-scale assembly of such structures is very time-consuming. Here, we investigated the efficiency of superstructure assembly on surfaces using indirect cross-linking through low-complexity connector strands binding staple strand extensions, instead of connector strands binding to scaffold loops. Using single-molecule imaging techniques, including fluorescence microscopy and atomic force microscopy, we show that low sequence complexity connector strands allow formation of DNA origami superstructures on lipid membranes, with an order-of-magnitude enhancement in the assembly speed of superstructures. A number of effects, including suppression of DNA hairpin formation, high local effective binding site concentration, and multivalency are proposed to contribute to the acceleration. Thus, the use of low-complexity sequences for DNA origami higher-order assembly offers a very simple but efficient way of improving throughput in DNA origami design.