Fluorescent and bioluminescent calcium indicators with tuneable colors and 
affinities

Mertes, Nicole; Busch, Marvin; Huppertz, Magnus-Carsten; Hacker, Christina Nicole; Gürth, Clara-Marie; Kühn, Stefanie; Hiblot, Julien; Koch, Birgit; Johnsson, Kai

doi:10.1021/jacs.2c01465

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Fluorescent and bioluminescent calcium indicators with tuneable colors and affinities

MPS-Authors

/persons/resource/persons264416

Mertes, Nicole
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons267987

Busch, Marvin
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons267989

Huppertz, Magnus-Carsten
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons267991

Hacker, Christina Nicole
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons252960

Gürth, Clara-Marie
Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons264412

Kühn, Stefanie
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons212621

Hiblot, Julien
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons243203

Koch, Birgit
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons203696

Johnsson, Kai
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

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https://www.biorxiv.org/content/10.1101/2021.11.13.468356v1.full.pdf
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https://pubs.acs.org/doi/10.1021/jacs.2c01465
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Citation

Mertes, N., Busch, M., Huppertz, M.-C., Hacker, C. N., Gürth, C.-M., Kühn, S., et al. (2022). Fluorescent and bioluminescent calcium indicators with tuneable colors and affinities. Journal of the American Chemical Society, 144(15), 6928-6935. doi:10.1021/jacs.2c01465.

Cite as: https://hdl.handle.net/21.11116/0000-0009-A174-E

Abstract

We introduce a family of bright, rhodamine-based calcium indicators with tuneable affinities and colors. The indicators can be specifically localized to different cellular compartments and are compatible with both fluorescence and bioluminescence readouts through conjugation to HaloTag fusion proteins. Importantly, their increase in fluorescence upon localization enables no-wash live-cell imaging, which greatly facilitates their use in biological assays. Applications as fluorescent indicators in rat hippocampal neurons include the detection of single action potentials and of calcium fluxes in the endoplasmic reticulum (ER). Applications as bioluminescent indicators include the recording of the pharmacological modulation of nuclear calcium in high-throughput-compatible assays. The versatility and remarkable ease of use of these indicators make them powerful tools for bioimaging and bioassays.