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Journal Article

A New Generation of the IMAGIC Image Processing System

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Heel,  Marin van
Fritz Haber Institute, Max Planck Society;

/persons/resource/persons268165

Harauz,  George
Fritz Haber Institute, Max Planck Society;

/persons/resource/persons263748

Orlova,  Elena V.
Fritz Haber Institute, Max Planck Society;

/persons/resource/persons268167

Schmidt,  Ralf
Fritz Haber Institute, Max Planck Society;
Image Science Software GmbH;

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Citation

Heel, M. v., Harauz, G., Orlova, E. V., Schmidt, R., & Schatz, M. (1996). A New Generation of the IMAGIC Image Processing System. Journal of Structural Biology, 116(1), 17-24. doi:10.1006/jsbi.1996.0004.


Cite as: https://hdl.handle.net/21.11116/0000-0009-A960-C
Abstract
One of the aims of modern microscopy is to quantify two-, three-, or even four-dimensional phenomena in biology, medicine, and material sciences. The requirements imposed on software by such data processing are exemplified by the design considerations of the IMAGIC-5 software system. This system includes facilities for multivariate statistical analysis of large data sets, for correlation averaging of two-dimensional crystals, and for three-dimensional reconstruction of macromolecular structures. The molecules may be arranged as two-dimensional crystals, as helices, or as single particles with arbitrary pointgroup symmetry. IMAGIC's novel angular reconstitution approach allows for the rapid determination of three-dimensional structures of uncrystallized molecules to high resolution. The general organization, user interaction strategy, file structure, and extendibility of IMAGIC are discussed and illustrated with some practical examples.