Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Antigenic evolution of human influenza H3N2 neuraminidase is constrained by charge balancing


Nourmohammad,  Armita
Max Planck Research Group Statistical physics of evolving systems, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Wang, Y., Lei, R., Nourmohammad, A., & Wu, N. C. (2021). Antigenic evolution of human influenza H3N2 neuraminidase is constrained by charge balancing. eLife, 10: e72516. doi:10.7554/eLife.72516.

Cite as: https://hdl.handle.net/21.11116/0000-0009-BF75-D
As one of the main influenza antigens, neuraminidase (NA) in H3N2 virus has evolved extensively for more than 50 years due to continuous immune pressure. While NA has recently emerged as an effective vaccine target, biophysical constraints on the antigenic evolution of NA remain largely elusive. Here, we apply combinatorial mutagenesis and next-generation sequencing to characterize the local fitness landscape in an antigenic region of NA in six different human H3N2 strains that were isolated around 10 years apart. The local fitness landscape correlates well among strains and the pairwise epistasis is highly conserved. Our analysis further demonstrates that local net charge governs the pairwise epistasis in this antigenic region. In addition, we show that residue coevolution in this antigenic region is correlated with the pairwise epistasis between charge states. Overall, this study demonstrates the importance of quantifying epistasis and the underlying biophysical constraint for building a model of influenza evolution.