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Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing

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John,  Nora
Wehner Research Group, Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Kolb,  Julia
Wehner Research Group, Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Wehner,  Daniel
Wehner Research Group, Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;
Wehner Research Group, Guck Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;

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Citation

John, N., Kolb, J., & Wehner, D. (2022). Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing. STAR Protocols, 3(1), 101093. doi:10.1016/j.xpro.2021.101093.


Cite as: https://hdl.handle.net/21.11116/0000-0009-D07B-2
Abstract
Zebrafish regenerate their spinal cord after injury, both at larval and adult stages. Larval zebrafish have emerged as a powerful model system to study spinal cord injury and regeneration due to their high optical transparency for in vivo imaging, amenability to high-throughput analysis, and rapid regeneration time. Here, we describe a protocol for the mechanical transection of the larval zebrafish spinal cord, followed by whole-mount tissue processing for in situ hybridization and immunohistochemistry to elucidate principles of regeneration.