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A method for determining enzymatically hydrolyzable phosphate (EHP) in natural waters

MPS-Authors

Albrecht,  Dieter
Department Microbial Ecology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Overbeck,  Jürgen
Department Microbial Ecology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Citation

Chrost, R. J., Siuda, W., Albrecht, D., & Overbeck, J. (1986). A method for determining enzymatically hydrolyzable phosphate (EHP) in natural waters. Limnology and Oceanography, 31(3), 662-687.


Cite as: https://hdl.handle.net/21.11116/0000-0009-CECB-B
Abstract
A method for determining enzymatically hydrolyzable phosphate (EHP) in natural waters is described, based on the determination of released inorganic phosphate after the hydrolysis of organophosphoric esters by free, dissolved phosphohydrolases (mainly phosphatase) produced by the biota. The method gives higher values in highly eutrophic waters than the classical procedure of Strickland and Parsons; in samples from less eutrophic habitats the two methods give similar results. The new method is simple, accurate, and can be used in both freshwater and marine studies. It is particularly recommended for determination in hypereutrophic waters, when Pi concentrations exceed 25 µg P liter−1. Representative field data from the euphotic zone of seven lakes and two marine habitats are presented.