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Journal Article

Actomyosin-assisted pulling of lipid nanotubes from lipid vesicles and cells

MPS-Authors
/persons/resource/persons232747

Jahnke,  Kevin
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons268974

Maurer,  Stefan J.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons249566

Weber,  Cornelia
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons268976

Hernandez Bücher,  Jochen Estebano
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons268418

Schoenit,  Andreas
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons141821

D´Este,  Elisa
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons75354

Cavalcanti-Adam,  Elisabetta Ada
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons220391

Göpfrich,  Kerstin
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://pubs.acs.org/doi/10.1021/acs.nanolett.1c04254
(Any fulltext)

https://pubs.acs.org/doi/pdf/10.1021/acs.nanolett.1c04254
(Any fulltext)

https://pubs.acs.org/doi/10.1021/acs.nanolett.1c04254?goto=supporting-info
(Supplementary material)

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Citation

Jahnke, K., Maurer, S. J., Weber, C., Hernandez Bücher, J. E., Schoenit, A., D´Este, E., et al. (2022). Actomyosin-assisted pulling of lipid nanotubes from lipid vesicles and cells. Nano Letters, 22(3), 1145-1150. doi:10.1021/acs.nanolett.1c04254.


Cite as: https://hdl.handle.net/21.11116/0000-0009-E1C2-D
Abstract
Molecular motors are pivotal for intracellular transport as well as cell motility and have great potential to be put to use outside cells. Here, we exploit engineered motor proteins in combination with self-assembly of actin filaments to actively pull lipid nanotubes from giant unilamellar vesicles (GUVs). In
particular, actin filaments are bound to the outer GUV membrane and the GUVs are seeded on a heavy meromyosin-coated substrate. Upon addition of ATP, hollow lipid nanotubes with a length of tens of micrometer are pulled from single GUVs due to the motor activity. We employ the same mechanism to pull lipid nanotubes from different types of cells. We find that the length and number of nanotubes critically depends on the cell type, whereby suspension cells form bigger networks than adherent cells. This suggests that molecular machines can be used to exert forces on living cells to probe membrane-to-cortex attachment.