Dynamic observation of 2H labeled compounds in the human brain with 1H versus 
2H magnetic resonance spectroscopy at 9.4T

Ruhm, L; Ziegs, T; Wright, AM; Mathy, CS; Murali-Manohar, S; Dorst, J; Avdievich, N; Henning, A

doi:10.1101/2022.01.24.477582

Datensatz

DATENSATZ AKTIONENEXPORT

Zur Ablage hinzufügen

Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben

Preprint

Dynamic observation of 2H labeled compounds in the human brain with 1H versus 2H magnetic resonance spectroscopy at 9.4T

MPG-Autoren

/persons/resource/persons215122

Ruhm, L
Research Group MR Spectroscopy and Ultra-High Field Methodology, Max Planck Institute for Biological Cybernetics, Max Planck Society;

/persons/resource/persons215132

Ziegs, T
Research Group MR Spectroscopy and Ultra-High Field Methodology, Max Planck Institute for Biological Cybernetics, Max Planck Society;

/persons/resource/persons215127

Wright, AM
Research Group MR Spectroscopy and Ultra-High Field Methodology, Max Planck Institute for Biological Cybernetics, Max Planck Society;

/persons/resource/persons215115

Murali-Manohar, S
Research Group MR Spectroscopy and Ultra-High Field Methodology, Max Planck Institute for Biological Cybernetics, Max Planck Society;

/persons/resource/persons215104

Dorst, J
Research Group MR Spectroscopy and Ultra-High Field Methodology, Max Planck Institute for Biological Cybernetics, Max Planck Society;

/persons/resource/persons133464

Avdievich, N
Research Group MR Spectroscopy and Ultra-High Field Methodology, Max Planck Institute for Biological Cybernetics, Max Planck Society;

/persons/resource/persons84402

Henning, A
Research Group MR Spectroscopy and Ultra-High Field Methodology, Max Planck Institute for Biological Cybernetics, Max Planck Society;

Externe Ressourcen

https://www.biorxiv.org/content/10.1101/2022.01.24.477582v1.full.pdf
(beliebiger Volltext)

Volltexte (beschränkter Zugriff)

Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.

Volltexte (frei zugänglich)

Es sind keine frei zugänglichen Volltexte in PuRe verfügbar

Ergänzendes Material (frei zugänglich)

Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar

Zitation

Ruhm, L., Ziegs, T., Wright, A., Mathy, C., Murali-Manohar, S., Dorst, J., et al. (submitted). Dynamic observation of 2H labeled compounds in the human brain with 1H versus 2H magnetic resonance spectroscopy at 9.4T.

Zitierlink: https://hdl.handle.net/21.11116/0000-0009-E241-E

Zusammenfassung

The metabolic pathway of [6,6’-2H2]-labeled glucose was investigated with two different techniques. The first technique used direct detection of deuterium applying Deuterium Metabolic Imaging (DMI). The second technique used the indirect detection of deuterium with proton MR spectroscopy (MRS) called Quantitative Exchanged-label Turnover (QELT) MRS. For the first time, time-resolved data was acquired for both techniques in the same healthy human subjects and directly compared. The time-curves were used in a kinetic model to estimate rates of the metabolic pathway of glucose. Two different kinetic models were compared. One included only DMI data, the second one combined DMI and QELT. For the first model, a tricarboxylic acid (TCA) cycle rate of 0.69 ± 0.10 μmol·min-1·g-1 was determined. For the second model, the estimated TCA cycle rate was 0.68 ± 0.12 μmol·min-1·g-1. In addition, the rate of glutamine synthesis from glutamate could be estimated with model 2 (0.51 ± 0.15 μmol·min-1·g-1). The sensitivity of both methods was evaluated and compared to alternative techniques.