Production of levan from Bacillus subtilis var. natto and apoptotic effect on 
SH-SY5Y neuroblastoma cells

Vieira, A. M.; Zahed, F.; Crispim, A. C.; de Souza Bento, E.; de Freitas Oliveira França, R.; Pinheiro, I. O.; Pardo, L. A.; Carvalho, B. M.

doi:10.1016/j.carbpol.2021.118613

Datensatz

DATENSATZ AKTIONENEXPORT

Zur Ablage hinzufügen

Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben

Zeitschriftenartikel

Production of levan from Bacillus subtilis var. natto and apoptotic effect on SH-SY5Y neuroblastoma cells

MPG-Autoren

/persons/resource/persons182494

Zahed, F.
Oncophysiology, Molecular biology of neuronal signals, Max Planck Institute of Experimental Medicine, Max Planck Society;

/persons/resource/persons182344

Pardo, L. A.
Oncophysiology, Molecular biology of neuronal signals, Max Planck Institute of Experimental Medicine, Max Planck Society;

Externe Ressourcen

Es sind keine externen Ressourcen hinterlegt

Volltexte (beschränkter Zugriff)

Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.

Volltexte (frei zugänglich)

Es sind keine frei zugänglichen Volltexte in PuRe verfügbar

Ergänzendes Material (frei zugänglich)

Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar

Zitation

Vieira, A. M., Zahed, F., Crispim, A. C., de Souza Bento, E., de Freitas Oliveira França, R., Pinheiro, I. O., et al. (2021). Production of levan from Bacillus subtilis var. natto and apoptotic effect on SH-SY5Y neuroblastoma cells. Carbohydrate Polymers, 273: 118613. doi:10.1016/j.carbpol.2021.118613.

Zitierlink: https://hdl.handle.net/21.11116/0000-000A-20A6-6

Zusammenfassung

Levan is a high-valued polysaccharide of fructose produced by several microbial species. These polysaccharides have been described as effective therapeutic agents in some human disease conditions, such as cancer, heart diseases and diabetes. The objective of this study was to examine the effect of levan (β-(2 → 6)-fructan) produced through sucrose fermentation by B. subtilis var. natto on the proliferation rate, cytotoxicity, and apoptosis of human neuroblastoma SH-SY5Y cells. It was obtained 41.44 g/L of levan in 18 h by biotechnological fermentation and SH-SY5Y cells were exposed to 1000 μg/mL of levan. The treatment with 1000 μg/mL of levan induced apoptosis in SH-SY5Y cancer cells by the significant increase in Annexin V/7-AAD and caspase 3/7 activation, but did not decrease proliferation or triggered a cytotoxic effect. 1000 μg/mL levan treatment is a promising therapeutic strategy for SH-SY5Y neuroblastoma cells.