A fluorescence microscopy-based protocol for volumetric measurement of 
lysolecithin lesion-associated de- and re-myelination in mouse brain

Bosch-Queralt, Mar; Tiwari, Vini; Damkou, Alkmini; Vaculciakova, Lenka; Alexopoulos, Ioannis; Simons, Mikael

doi:10.1016/j.xpro.2022.101141

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A fluorescence microscopy-based protocol for volumetric measurement of lysolecithin lesion-associated de- and re-myelination in mouse brain

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Vaculciakova, Lenka
Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society;

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Citation

Bosch-Queralt, M., Tiwari, V., Damkou, A., Vaculciakova, L., Alexopoulos, I., & Simons, M. (2022). A fluorescence microscopy-based protocol for volumetric measurement of lysolecithin lesion-associated de- and re-myelination in mouse brain. STAR Protocols, 3(1): 101141. doi:10.1016/j.xpro.2022.101141.

Cite as: https://hdl.handle.net/21.11116/0000-0009-EFDF-0

Abstract

Lysolecithin injections into the white matter tracts of the central nervous system are a valuable tool to study remyelination, but evaluating the resulting demyelinating lesion size is challenging. Here, we present a protocol to consistently measure the volume of demyelination and remyelination in mice following brain lysolecithin injections. We describe serial sectioning of the lesion, followed by the evaluation of the demyelinated area in two-dimensional images. We then detail the computation of the volume using our own automated iPython script.