English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

A fluorescence microscopy-based protocol for volumetric measurement of lysolecithin lesion-associated de- and re-myelination in mouse brain

MPS-Authors
/persons/resource/persons198956

Vaculciakova,  Lenka
Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

BoschQueralt_2022.pdf
(Publisher version), 5MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Bosch-Queralt, M., Tiwari, V., Damkou, A., Vaculciakova, L., Alexopoulos, I., & Simons, M. (2022). A fluorescence microscopy-based protocol for volumetric measurement of lysolecithin lesion-associated de- and re-myelination in mouse brain. STAR Protocols, 3(1): 101141. doi:10.1016/j.xpro.2022.101141.


Cite as: https://hdl.handle.net/21.11116/0000-0009-EFDF-0
Abstract
Lysolecithin injections into the white matter tracts of the central nervous system are a valuable tool to study remyelination, but evaluating the resulting demyelinating lesion size is challenging. Here, we present a protocol to consistently measure the volume of demyelination and remyelination in mice following brain lysolecithin injections. We describe serial sectioning of the lesion, followed by the evaluation of the demyelinated area in two-dimensional images. We then detail the computation of the volume using our own automated iPython script.