Cell cycle-related changes in the conducting properties of r-eag K+ channels

Pardo, L. A.; Bruggemann, A.; Camacho, J.; Stuhmer, W.

doi:10.1083/jcb.143.3.767

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学術論文

Cell cycle-related changes in the conducting properties of r-eag K+ channels

MPS-Authors

Pardo, L. A.
Max Planck Society;

Bruggemann, A.
Max Planck Society;

Camacho, J.
Max Planck Society;

Stuhmer, W.
Max Planck Society;

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https://www.ncbi.nlm.nih.gov/pubmed/9813096
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引用

Pardo, L. A., Bruggemann, A., Camacho, J., & Stuhmer, W. (1998). Cell cycle-related changes in the conducting properties of r-eag K+ channels. J Cell Biol, 143(3), 767-75. doi:10.1083/jcb.143.3.767.

引用: https://hdl.handle.net/21.11116/0000-0009-F215-E

要旨

Release from arrest in G2 phase of the cell cycle causes profound changes in rat ether-a-go-go (r-eag) K+ channels heterologously expressed in Xenopus oocytes. The most evident consequence of the onset of maturation is the appearance of rectification in the r-eag current. The trigger for these changes is located downstream of the activation of mitosis-promoting factor (MPF). We demonstrate here that the rectification is due to a voltage-dependent block by intracellular Na+ ions. Manipulation of the intracellular Na+ concentration indicates that the site of Na+ block is located approximately 45% into the electrical distance of the pore and is only present in oocytes undergoing maturation. Since the currents through excised patches from immature oocytes exhibited a fast rundown, we studied CHO-K1 cells permanently transfected with r-eag. These cells displayed currents with a variable degree of block by Na+ and variable permeability to Cs+. Partial synchronization of the cultures in G0/G1 or M phases of the cell cycle greatly reduced the variability. The combined data obtained from mammalian cells and oocytes strongly suggest that the permeability properties of r-eag K+ channels are modulated during cell cycle-related processes.