Cryo-electron microscopy of the surface protein of Sulfolobus shibatae

Lembcke, Gerald; Baumeister, Wolfgang; Zemlin, Friedrich; Beckmann, Erich

doi:10.1016/0304-3991(93)90245-S

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Cryo-electron microscopy of the surface protein of Sulfolobus shibatae

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Zemlin, Friedrich
Fritz Haber Institute, Max Planck Society;

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Beckmann, Erich
Fritz Haber Institute, Max Planck Society;

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Citation

Lembcke, G., Baumeister, W., Zemlin, F., & Beckmann, E. (1993). Cryo-electron microscopy of the surface protein of Sulfolobus shibatae. Ultramicroscopy, 49(1-4), 397-406. doi:10.1016/0304-3991(93)90245-S.

Cite as: https://hdl.handle.net/21.11116/0000-000A-0CF8-2

Abstract

Using cryo-microscopy at liquid-helium temperature we have determined the projection structure of the surface layer of the archaebacterium Sulfolobus shibatae embedded in aurothioglucose to a resolution of 0.85 nm. Image analysis was performed in real space and incorporated a classification of unit cells, thus taking into account the frequent occurrence of twin boundaries. In an attempt to identify resolution-limiting factors we have performed a distortion analysis quantifying the main distortion parameters.