Structure and biosynthesis of deoxy-polyamine in Xenorhabdus bovienii

Wenski, Sebastian L.; Berghaus, Natalie; Keller, Nadine; Bode, Helge B.

doi:10.1093/jimb/kuab006

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Structure and biosynthesis of deoxy-polyamine in Xenorhabdus bovienii

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Bode, Helge B.
Natural Product Function and Engineering, Department of Natural Products in Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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https://doi.org/10.1093/jimb/kuab006
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Citation

Wenski, S. L., Berghaus, N., Keller, N., & Bode, H. B. (2021). Structure and biosynthesis of deoxy-polyamine in Xenorhabdus bovienii. Journal of Industrial Microbiology & Biotechnology, 48(3-4): kuab006. doi:10.1093/jimb/kuab006.

Cite as: https://hdl.handle.net/21.11116/0000-000A-0414-B

Abstract

Polyamine moieties have been described as part of the fabclavine and zeamine family of natural products. While the corresponding biosynthetic gene clusters have been found in many different proteobacteria, a unique BGC was identified in the entomopathogenic bacterium Xenorhabdus bovienii. Mass spectrometric analysis of a X. bovienii mutant strain revealed a new deoxypolyamine. The corresponding biosynthesis includes two additional reductive steps, initiated by an additional dehydratase (DH) domain, which was not found in any other Xenorhabdus strain. Moreover, this DH domain could be successfully integrated into homologous biosynthesis pathways, leading to the formation of other deoxy-polyamines. Additional heterologous production experiments revealed that the DH domain could act in cis as well as in trans.