A New Docking Domain Type in the Peptide-Antimicrobial-Xenorhabdus Peptide 
Producing Nonribosomal Peptide Synthetase from Xenorhabdus bovienii

Watzel, Jonas; Hacker, Carolin; Duchardt-Ferner, Elke; Bode, Helge B.; Woehnert, Jens

doi:10.1021/acschembio.9b01022

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A New Docking Domain Type in the Peptide-Antimicrobial-Xenorhabdus Peptide Producing Nonribosomal Peptide Synthetase from Xenorhabdus bovienii

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Bode, Helge B.
Natural Product Function and Engineering, Department of Natural Products in Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Goethe-Universität Frankfurt am Main, External Organizations;

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Citation

Watzel, J., Hacker, C., Duchardt-Ferner, E., Bode, H. B., & Woehnert, J. (2020). A New Docking Domain Type in the Peptide-Antimicrobial-Xenorhabdus Peptide Producing Nonribosomal Peptide Synthetase from Xenorhabdus bovienii. ACS CHEMICAL BIOLOGY, 15(4), 982-989. doi:10.1021/acschembio.9b01022.

Cite as: https://hdl.handle.net/21.11116/0000-000A-0514-A

Abstract

Nonribosomal peptide synthetases (NRPSs) produce a wide variety of different natural products from amino acid precursors. In contrast to single protein NRPS, the NRPS of the bacterium Xenorhabdus bovienii producing the peptide-antimicrobial-Xenorhabdus (PAX) peptide consists of three individual proteins (PaxA/B/C), which interact with each other noncovalently in a linear fashion. The specific interactions between the three different proteins in this NRPS system are mediated by short C- and N-terminal docking domains ((C/N)DDs). Here, we investigate the structural basis for the specific interaction between the C DD from the protein PaxB and the (DD)-D-N from PaxC. The isolated DD peptides feature transient alpha-helical conformations in the absence of the respective DD partner. Isothermal titration calorimetry (ITC) and nuclear magnetic resonance (NMR) titration experiments showed that the two isolated DDs bind to each other and form a structurally well-defined complex with a dissociation constant in the micromolar range as is typical for many DD interactions. Artificial linking of this DD pair via a flexible glycine-serine (GS) linker enabled us to solve the structure of the DD complex by NMR spectroscopy. In the complex, the two DDs interact with each other by forming a three helix bundle arranged in an overall coiled-coil motif. Key interacting residues were identified in mutagenesis experiments. Overall, our structure of the PaxB (DD)-D-C/PaxC (DD)-D-N complex represents an architecturally new type of DD interaction motif.