Reprogramming Promiscuous Nonribosomal Peptide Synthetases for Production of 
Specific Peptides

Cai, Xiaofeng; Zhao, Lei; Bode, Helge B.

doi:10.1021/acs.orglett.9b00395

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Reprogramming Promiscuous Nonribosomal Peptide Synthetases for Production of Specific Peptides

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Citation

Cai, X., Zhao, L., & Bode, H. B. (2019). Reprogramming Promiscuous Nonribosomal Peptide Synthetases for Production of Specific Peptides. ORGANIC LETTERS, 21(7), 2116-2120. doi:10.1021/acs.orglett.9b00395.

Cite as: https://hdl.handle.net/21.11116/0000-000A-05D2-3

Abstract

Pairs of docking domains (DDs) mediate the selective interations between adjacent nonribosomal peptide synthetases (NRPSs) to form defined protein-protein interactions resulting in defined peptide sequences. New specific rhabdopeptide/xenortide-like peptides (RXPs) were generated by swapping of either flexible or nonfunctional DD pairs between these monomodular RXP-NRPSs against DDs from collinear NRPSs. The results presented a promising means of engineering RXP-producing NRPSs to obtain desired peptides and further substantiated the decisive role of DDs in the NRP synthesis.