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Journal Article

Natural Product Diversification Mediated by Alternative Transcriptional Starting

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Tobias, N. J., Linck, A., & Bode, H. B. (2018). Natural Product Diversification Mediated by Alternative Transcriptional Starting. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 57(20), 5699-5702. doi:10.1002/anie.201713199.

Cite as: https://hdl.handle.net/21.11116/0000-000A-047B-8
Photorhabdus luminescens dedicates a significant proportion of its genome to the production of natural products. These products and the structural variation in their derivatives may occur by a number of well-described mechanisms, such as module skipping or precursor promiscuity. Cappable-seq was used to identify transcriptional start sites of many of the gene clusters present in P.luminescens TTO1. We discovered that variations associated with the non-ribosomal peptide synthetase Kol, which is responsible for kolossin A production, possessed a number of internal transcripts that lead to synthesis of the smaller kolossin derivatives kolossin B and C. The data here support a new mechanism of natural product biosynthetic variation whereby mRNA may code for shorter NRPS enzymes in addition to full-length proteins, resulting in the production of smaller peptide derivatives.