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Book Chapter

Chapter Eight - A fast-track protocol for protein expression using the BEV system

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Scholz,  Judith
Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society;

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Suppmann,  Sabine
Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Scholz, J., & Suppmann, S. (2021). Chapter Eight - A fast-track protocol for protein expression using the BEV system. In W. B. O’Dell, & Z. Kelman (Eds.), Recombinant Protein Expression: Eukaryotic Hosts (pp. 171-190). London: Academic Pr.


Cite as: https://hdl.handle.net/21.11116/0000-000A-23A4-5
Abstract
Baculovirus-insect cell expression (BEV) has become one of the most widely used eukaryotic systems for heterologous protein expression. The combination of engineered baculovirus genomes together with a variety of compatible vectors, robust insect cell lines, serum-free media and commercial kits have made it a standard workhorse in many "non-virology-expert" laboratories. Despite these significant improvements, the BEV system still has major drawbacks, primarily the time required to amplify recombinant virus and its inherent instability. Here we present an easy-to-adopt simplified and shortened protocol.