Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Changes in DNA synthesis and cell proliferation during hepatocarcinogenesis by diethylnitrosamine


Rajewsky,  Manfred F.
Max Planck Institute of Biophysics, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Rajewsky, M. F. (1967). Changes in DNA synthesis and cell proliferation during hepatocarcinogenesis by diethylnitrosamine. European Journal of Cancer, 3(4-5), 335-342. doi:10.1016/0014-2964(67)90016-3.

Cite as: https://hdl.handle.net/21.11116/0000-000A-53FB-E
In the context of a general study on the regulation of proliferation in mammalian cell populations during carcinogenesis, DNA synthesis and proliferative activity in rat liver was investigated during carcinogenesis by continuous administration of diethylnitrosamine. This carcinogenic system is characterised by an extremely high and liver specific tumour yield and very precise dose response relationships (median time of death with multifocal hepatocellular carcinomata: 160 days±6% S.D.). Autoradiographic determination of 3H-thymidine labelling indices (L.I.) after in vivo pulse exposure to 3H-Tdr at several stages of carcinogenesis was combined with in vitro measurements of 3H-Tdr incorporation into fresh liver explants under standardised conditions. Whereas the proliferative activities of the non-parenchymal cell populations remained unaltered during carcinogenesis, 3H-Tdr incorporation and L.I. of the parenchymal cell population showed characteristic changes. These are analysed and discussed in relation to the interference of the carcinogen with mechanisms controlling liver cell proliferation.