Mechanism of spindle pole organization and instability in human oocytes

So, C.; Menelaou, K.; Uraji, J.; Harasimov, K.; Steyer, A. M.; Seres, K. B.; Bucevičius, J.; Lukinavicius, G.; Möbius, W.; Sibold, C.; Tandler-Schneider, A.; Eckel, H.; Moltrecht, R.; Blayney, M.; Elder, K.; Schuh, M.

doi:10.1126/science.abj3944

Datensatz

DATENSATZ AKTIONENEXPORT

Zur Ablage hinzufügen

Bitte beachten Sie, dass eine neuere Version dieses Datensatzes verfügbar ist:
https://pure.mpg.de/pubman/item/item_3375936_2

DetailsÜbersicht

Freigegeben

Zeitschriftenartikel

Mechanism of spindle pole organization and instability in human oocytes

MPG-Autoren

/persons/resource/persons226224

So, C.
Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons273649

Menelaou, K.
Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons227938

Uraji, J.
Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons217935

Harasimov, K.
Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons229531

Steyer, A. M.
Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

Bucevičius, J.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

Lukinavicius, G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons182306

Möbius, W.
Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons188399

Schuh, M.
Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

Externe Ressourcen

Es sind keine externen Ressourcen hinterlegt

Volltexte (beschränkter Zugriff)

Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.

Volltexte (frei zugänglich)

Es sind keine frei zugänglichen Volltexte in PuRe verfügbar

Ergänzendes Material (frei zugänglich)

Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar

Zitation

So, C., Menelaou, K., Uraji, J., Harasimov, K., Steyer, A. M., Seres, K. B., et al. (2022). Mechanism of spindle pole organization and instability in human oocytes. Science, 375(6581): eabj3944. doi:10.1126/science.abj3944.

Zitierlink: https://hdl.handle.net/21.11116/0000-000A-6C08-5

Zusammenfassung

Human oocytes are prone to assembling meiotic spindles with unstable poles, which can favor aneuploidy in human eggs. The underlying causes of spindle instability are unknown. We found that NUMA (nuclear mitotic apparatus protein)–mediated clustering of microtubule minus ends focused the spindle poles in human, bovine, and porcine oocytes and in mouse oocytes depleted of acentriolar microtubule-organizing centers (aMTOCs). However, unlike human oocytes, bovine, porcine, and aMTOC-free mouse oocytes have stable spindles. We identified the molecular motor KIFC1 (kinesin superfamily protein C1) as a spindle-stabilizing protein that is deficient in human oocytes. Depletion of KIFC1 recapitulated spindle instability in bovine and aMTOC-free mouse oocytes, and the introduction of exogenous KIFC1 rescued spindle instability in human oocytes. Thus, the deficiency of KIFC1 contributes to spindle instability in human oocytes.