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Book Chapter

Live-cell imaging of RNA transport in axons of cultured primary neurons

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Hees,  J. Tabitha
Max Planck Research Group: Neurometabolism / Harbauer, MPI of Neurobiology, Max Planck Society;

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Harbauer,  Angelika B.
Max Planck Research Group: Neurometabolism / Harbauer, MPI of Neurobiology, Max Planck Society;

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Citation

Hees, J. T., & Harbauer, A. B. (2022). Live-cell imaging of RNA transport in axons of cultured primary neurons. In A. Vagnoni (Ed.), Axonal Transport. Methods and Protocols (1, pp. 225-237). New York: Humana.


Cite as: https://hdl.handle.net/21.11116/0000-000A-533A-8
Abstract
The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging.