Structure elucidation of the blood group B like and blood group I active 
octaantennary ceramide tetracontasaccharide from rabbit erythrocyte membranes 
by two-dimensional proton NMR spectroscopy at 600 MHz

Dabrowski, Janusz; Dabrowski, Ursula; Bermel, Wolfgang; Kordowicz, Maria; Hanfland, and Peter

doi:10.1021/bi00414a030

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Structure elucidation of the blood group B like and blood group I active octaantennary ceramide tetracontasaccharide from rabbit erythrocyte membranes by two-dimensional proton NMR spectroscopy at 600 MHz

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Dabrowski, Janusz
Department of Organic Chemistry, Max Planck Institute for Medical Research, Max Planck Society;

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Dabrowski, Ursula
Department of Organic Chemistry, Max Planck Institute for Medical Research, Max Planck Society;

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引用

Dabrowski, J., Dabrowski, U., Bermel, W., Kordowicz, M., & Hanfland, a. P. (1988). Structure elucidation of the blood group B like and blood group I active octaantennary ceramide tetracontasaccharide from rabbit erythrocyte membranes by two-dimensional proton NMR spectroscopy at 600 MHz. Biochemistry, 27(14), 5149-5155. doi:10.1021/bi00414a030.

引用: https://hdl.handle.net/21.11116/0000-000A-54AC-6

要旨

The primary structure of the ceramide tetracontasaccharide (1) from rabbit erythrocyte
membranes has been determined with the aid of 600-MHz two-dimensional phase-sensitive correlated, “totally
correlated” (TOCSY, homonuclear Hartmann-Hahn), relayed coherence transfer, triple quantum filtered,
and nuclear Overhauser enhancement NMR spectra. It was shown that obtaining subspectra of the
constituent sugar residues from a totally correlated spectrum and assigning the resonances occurring in these
subspectra by analyzing the relevant cross-peaks in phase-sensitive correlated spectra is the most efficient
way for establishing complex oligosaccharide structures. This analysis has shown 1 to be the highest
homologue of the multiantennary neolactoglycosphingolipids of the following general formula with = 7:
Galo,1-3Gal01- 4GlcNAc01-3Gal/31
6
Gala1-3Gal/31-4GlcNAC/3