Mining ancient microbiomes using selective enrichment of damaged DNA molecules

Weiß, CL; Gansauge, M-T; Aximu-Petri, A; Meyer, M; Burbano, HA

doi:10.1186/s12864-020-06820-7

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Mining ancient microbiomes using selective enrichment of damaged DNA molecules

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Weiß, CL
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;
Research Group for Ancient Genomics and Evolution, Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Burbano, HA
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;
Research Group for Ancient Genomics and Evolution, Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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引用

Weiß, C., Gansauge, M.-T., Aximu-Petri, A., Meyer, M., & Burbano, H. (2020). Mining ancient microbiomes using selective enrichment of damaged DNA molecules. BMC Genomics, 21(1):. doi:10.1186/s12864-020-06820-7.

引用: https://hdl.handle.net/21.11116/0000-000A-5708-C

要旨

The identification of bona fide microbial taxa in microbiomes derived from ancient and historical samples is complicated by the unavoidable mixture between DNA from ante- and post-mortem microbial colonizers. One possibility to distinguish between these sources of microbial DNA is querying for the presence of age-associated degradation patterns typical of ancient DNA (aDNA). The presence of uracils, resulting from cytosine deamination, has been detected ubiquitously in aDNA retrieved from diverse sources, and used as an authentication criterion. Here, we employ a library preparation method that separates molecules that carry uracils from those that do not for a set of samples that includes Neandertal remains, herbarium specimens and archaeological plant remains.